Assessment of Cell Disruption and Carotenoids Extraction from Sporidiobolus salmonicolor (CBS 2636) |
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Authors: | Eunice Valduga Alexsandra Valério Pihetra Oliveira Tatsch Helen Treichel Agenor Furigo Jr Marco Di Luccio |
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Affiliation: | (1) Department of Food Engineering, URI, Campus de Erechim, Av. Sete de Setembro 1621, Erechim, RS, 99700-000, Brazil;(2) Department of Chemistry and Food Engineering, UFSC, Florianópolis, SC, 88040-900, Brazil |
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Abstract: | The increasing demand for carotenoids by industries has drawn attention to their bio-production. Since pigments are intracellular,
extraction steps are then needed after cell cultivation. In this work, different strategies for extraction of carotenoid pigments
from Sporidiobolus salmonicolor (CBS 2636) were studied. Different solvents (dimethyl sulfoxide, petroleum ether, hexane, ethyl acetate, chloroform, and
acetone), liquid N2, and diatomaceous earth were used to disrupt the cell and thus release the intracellular carotenoids. The results of this
study showed that when multiple solvents were used, a synergistic effect on the extent of carotenoids recovery was obtained.
Maximum concentration of total carotenoids (913 μg/L) was obtained in the treatment using liquid N2 and dimethyl sulfoxide to disrupt the cell, followed by the extraction with a solution of acetone/methanol (7:3, v/v). |
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Keywords: | Recovery Carotenoids Sporidiobolus salmonicolor Disruption |
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