| 利用狭鳕加工废料制备胶原的表征及其生物学功能 |
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| 引用本文： | 刘寒,刘成圣,陈军,李相宜,范冰,陈丽媛,陈西广. 利用狭鳕加工废料制备胶原的表征及其生物学功能[J]. 功能材料, 2011, 42(5): 959-962 |
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| 作者姓名： | 刘寒 刘成圣 陈军 李相宜 范冰 陈丽媛 陈西广 |
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| 作者单位： | 刘寒,刘成圣,李相宜,范冰,陈丽媛,陈西广,LIU Han,LIU Cheng-sheng,LI Xiang-yi,FAN Bing,CHEN Li-yuan,CHEN Xi-guang(中国海洋大学,海洋生命学院,山东,青岛,266003);陈军,CHEN Jun(青岛商业职工医院外科部,山东,青岛,266011) |
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| 基金项目： | 国际合作NSFC-KOSEF资助项目，ISTCP资助项目 |
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| 摘 要： | 以狭鳕加工废料为来源提取酸溶胶原并加以表征.该胶原在230nm处有最大紫外吸收,红外吸收光谱指示存在螺旋结构.电泳图谱分离出多个亚基,鉴定为Ⅰ型胶原.该胶原有独特的氨基酸组成,甘氨酸达19.8%,羟脯氨酸占6.9%.酸水解制备的胶原多肽平均分子量区间为3.2～53kDa.胶原多肽具有不同的生物学功能:较高分子量的胶原多...
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| 关 键 词： | 狭鳕 胶原多肽 表征 分子量 生物学功能 |
Preparation and characterization and biological functions of collagen from alaska pollock processing by-products |
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| LIU Han,LIU Cheng-sheng,CHEN Jun,LI Xiang-yi,FAN Bing,CHEN Li-yuan,CHEN Xi-guang. Preparation and characterization and biological functions of collagen from alaska pollock processing by-products[J]. Journal of Functional Materials, 2011, 42(5): 959-962 |
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| Authors: | LIU Han LIU Cheng-sheng CHEN Jun LI Xiang-yi FAN Bing CHEN Li-yuan CHEN Xi-guang |
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| Affiliation: | LIU Han1,LIU Cheng-sheng1,CHEN Jun2,LI Xiang-yi1,FAN Bing1,CHEN Li-yuan1,CHEN Xi-guang1(1.College of Marine Life Science,Ocean University of China,Qingdao 266003,China,2.Department of Surgery,The Commercial Staff & Workers Hospital of Qingdao,Qingdao 266011,China) |
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| Abstract: | Acid-soluble collagen from the processing waste of Alaska pollock was extracted and characterized.It had a maximum UV absorbance at 230nm while amino acid composition and SDS-PAGE suggested it was type I collagen.FTIR spectra showed the existence of helical arrangements.Hydrolysates were prepared by acid hydrolysis.The average molecular weights of its hydrolysates ranged from 3.2 to 53kDa.Functional properties of the collagen have been diversified by hydrolysis.For the collagen polypeptides of higher molecu... |
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| Keywords: | alaska pollock collagen polypeptide characterization molecular weight biological functions |
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