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Protein oriented ligation on nanoparticles exploiting O6-alkylguanine-DNA transferase (SNAP) genetically encoded fusion |
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| Authors: | Colombo Miriam Mazzucchelli Serena Montenegro Josè Maria Galbiati Elisabetta Corsi Fabio Parak Wolfgang J Prosperi Davide |
| Affiliation: | Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, Milan, Italy. |
| Abstract: | A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the site-specific covalent conjugation of targeting peptides to nanoparticles. An scFv mutant selective for HER2 tumor antigen is chosen as the targeting ligand. SNAP-scFv is immobilized on magnetofluorescent nanoparticles and its targeting efficiency against HER2-positive cells is assessed by flow cytometry and immunofluorescence. |
| Keywords: | magnetic nanoparticles magnetofluorescence fusion proteins bioconjugation tumor targeting |
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