基于福尔马肼溶液的CRP快速分析仪检测方法

C-反应蛋白(C-reactive protein,CRP)快速分析仪是基层医疗机构常用的快速检验仪器之一,目前尚未有相应的规程和规范对此类型仪器的量值溯源和可靠性做为依据。通过分析福尔马肼溶液和C-反应蛋白溶液分子尺寸和分子量分布,依据散射原理并参照JJG 880-2006浊度计的方法项目对该仪器进行测试,并对方法的合理性进行评估。实验选用常见的Orion厂商的Quikread101(速率散射法)CRP快速分析仪,表明在浊度范围为(675～1000)NTU的福尔马肼溶液系列溶液测试时,测定线性及重复性良好。通过对系列福尔马肼溶液与C反应蛋白系列溶液进行对比,找到对应的福尔马肼溶液的浊度值,为该类仪器量值溯源提供了一种参考方法。

Method of CRP Rapid Analyzer Based on Formazine Polymer

C-reactive protein (CRP) rapid analyzer is one of the commonly used rapid testing instruments in primary medical institutions. At present, there are no relevant regulations and specifications for traceability and reliability of this type of instrument. By analyzing the molecular size and molecular weight distribution of formazine solution and C-reactive protein solution, the instrument was tested on the basis of scattering principle and referring to the method items of JJG 880-2006 turbidimeter, and the rationality of the method was evaluated. Quikread101 (Rate Scattering) CRP rapid analyzer from Orion manufacturer was used in the experiment. The results showed that the linearity and repeatability of the determination were good when the formazine solution series was tested in turbidity range of (675~1 000) NTU. By comparing a series of formazine solutions with the series of C-reactive protein solutions, the corresponding turbidity values of formazine solutions were found, which provided a reference method for tracing the values of these instruments.