柿叶提取物体外抑瘤作用初步研究

目的:研究柿叶提取物在体外对肿瘤细胞的抑制作用。方法:采用不同方法提取柿子叶有效部位,用MTT法检测其对Hela(人宫颈癌细胞)和H22(鼠肝癌细胞)两种细胞的生长抑制率,并用SPSS进行统计分析。结果:柿叶水、醇提取物作用于Hela细胞,具有明显的抑瘤效应;柿叶水、醇提取物作用于H22细胞,有明显的抑瘤效应。加药后肿瘤细胞在形态以及生长方式上面发生明显改变。结论:柿叶不同提取物在一定浓度下对Hela细胞和H22细胞均有明显的抑制增殖作用。

Primary Study on Anti-tumor Effects of Extract of Leaves of Persimmon in vitro

Objective: To study the inhibitive effect of the active part of the leaves of persimmon on tumor cell lines. Methods: MTT (methylthiazolydiphenyl-tetrazolium bromide) method was taken to detect the anti-tumor activities of the acitve part of the leaves of persimmon after it was extracted from the leaves on Hela and H22 cell lines. And the data was analyzed by SPSS. Results: ①The extract of the leaves of persimmon with water and ethanol had evident inhibition effect on Hela’s growth. ②The extract of the leaves of persimmon with water and ethanol had evident inhibition effect on H22’s growth. ③The extract can change the cellular morphology and growth mode. Conclusion: The extract of leaves of persimmon had evident inhibition effect on Hela and H22 cell in a certain dosage.