学生宿舍和食堂环境中克罗诺杆菌污染状况及分子分型和耐药特征分析

目的 了解徐州市某学校学生宿舍和食堂环境中克罗诺杆菌污染状况、分子分型及耐药特征,为预警食源性克罗诺杆菌病暴发提供参考.方法 采集徐州市某学校学生宿舍和食堂环境样品156份,分离并鉴定克罗诺杆菌,并利用基于O-抗原的血清分型和多位点序列分型(multilocus sequence typing,MLST)技术对分离株进...

Contamination status, molecular typing and antimicrobial resistance of Cronobacter spp. in dormitory and canteen

Objective To investigate the contamination status, molecular typing and antimicrobial resistance characteristics of Cronobacter spp. in dormitory and canteen of a school in Xuzhou, and to provide reference data for early-warning of foodborne disease outbreak caused by Cronobacter spp.. Methods A total of 156 environmental samples were collected from the dormitory and canteen of a school in Xuzhou, and Cronobacter strains were isolated and identified. The O-antigen-based serotyping and multilocus sequence typing were applied for molecular typing of the Cronobacter strains, and the antimicrobial resistance of Cronobacter strains were determined by disk diffusion method. Results Eleven (7.1%) of 156 environmental samples were positive for Cronobacter spp., with the detection rate of 6.6%(8/121) and 8.6%(3/35) in dormitories and canteens, respectively. Based on the fusA sequence analysis, 8 of 11 isolates were identified as C.sakazakii, whereas the other 3 were identified as C.malonaticus. The 11 Cronobacter strains were assigned to 5 sequence types, and ST500 was the predominant sequence type (54.5%, 6/11). Meanwhile, the 11 Cronobacter strains were divided into 5 serotypes, with C.sakazakii serotype O7 (Csak O7) being the predominant serotype (54.5%, 6/11). The antimicrobial resistance test indicated that all isolates were sensitive to cefixime, amikacin, ciprofloxacin, furantoin, chloramphenicol and ticarcillin/clavulanic acid, whereas strains resistant to cefthiophene (63.6%, 7/11) and meropenem (27.3%, 3/11) were founded. Conclusion There is a risk of contamination of Cronobacter spp. in the environment of student dormitory and canteen. The epidemiological surveillance of Cronobacter spp. in the dormitory and canteen environment should be strengthened in future.