美味牛肝菌多糖对急性肝损伤小鼠的保肝作用

用不同剂量的美味牛肝菌多糖(Boletus edulis polysaccharides,BEP)给予小鼠灌胃连续30d后,按10mL/kg剂量腹腔注射含体积分数0.2%CCl4的花生油溶液,建立CCl4诱导小鼠肝损伤模型。测定肝脏指数、肝脏重量、血清谷丙转氨酶(ALT)、谷草转氨酶(AST),以及肝组织中超氧化物岐化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)及还原型谷胱甘肽(GSH)水平,并进一步观察肝脏组织病理学变化。结果表明:BEP能显著抑制CCl4急性肝损伤小鼠肝脏指数、血清ALT、AST水平以及肝脏MDA含量的升高,提高肝脏GSH-Px、SOD及GSH活性,其中800 mg/(kg BW獉d)剂量水平效果最佳,BEP对CCl4急性肝损伤小鼠有一定的保护作用。

Effect of Boletus edulis polysaccharides on liver protection to CCl4-induced acute hepatic damage mice

The mice in the treatment group were administrated with the BEP at doses of 200, 400, and 800 mg/(kg BW·d) for 30 consecutive days. The liver injury model was established by intraperitoneal injection of CCl₄. The alanine transaminase (ALT), aspartate transaminase (AST) contents in serum, and superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and glutathione (GSH) levels in liver were measured. Meanwhile, a hepatic pathological examination was conducted. The results of this study showed that Boletus edulis polysaccharides could, to some extent, protect mice from CCl₄-induced liver injury. In addition, Boletus edulis polysaccharides significantly prevent the increase in liver index spleen index, serum ALT, AST, and liver MDA levels, caused by CCl₄ in mice. Boletus edulis polysaccharides could also markedly increase the activities of liver SOD, GSH-Px, GSH. Among the tested doses, 800 mg/(kg BW·d) showed the best effect. Histopathological examinations further confirmed these results.