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| 二次接种叠加生物素的谷氨酸发酵工艺研究 | |
| 引用本文： | 邓毛程,梁世中,王瑶,朱明军.二次接种叠加生物素的谷氨酸发酵工艺研究[J].食品与发酵工业,2007,33(7):1-5. |
| 作者姓名： | 邓毛程 梁世中 王瑶 朱明军 |
| 作者单位： | 1. 华南理工大学生物科学与工程学院,广东,广州,510640;广东轻工职业技术学院,广东,广州,510300 2. 华南理工大学生物科学与工程学院,广东,广州,510640 3. 广州奥桑味精食品有限公司,广东,广州,510280 |
| 摘 要： | 由于受到发酵罐溶氧条件的限制,在高浓度生物素的谷氨酸发酵中往往出现产酸与糖酸转化率不协调的现象,针对这一现象,研究了二次接种叠加生物素的谷氨酸发酵工艺。在试验所用的发酵罐中,采用8.0μg/L生物素浓度的培养基作为发酵基础培养,经过一段时间发酵后,接入第二次种子液以及3.0μg/L(发酵液初始体积)的生物素量,通过适当的发酵控制,产酸水平达到139.6g/L,糖酸转化率高达62.80%,单罐谷氨酸产量比一次接种添加8.0μg/L生物素的发酵工艺提高了15.78%。 |
| 关 键 词： | 二次接种 叠加 生物素 谷氨酸 溶氧 |
| 修稿时间： | 2007-01-26 |
Study on Glutamic Acid Fermentation Process with Two-step Inoculations and Additions of Biotin |
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| Deng Maocheng,Liang Shizhong,Wang Yao,Zhu Mingjun.Study on Glutamic Acid Fermentation Process with Two-step Inoculations and Additions of Biotin[J].Food and Fermentation Industries,2007,33(7):1-5. | |
| Authors: | Deng Maocheng Liang Shizhong Wang Yao Zhu Mingjun |
| Affiliation: | 1College of Bioscience and Engineering, South China University of Technology, Guangzhou 510640,China;2Guangdong Industry Technical College, Guangzhou 510300,China;3Guangzhou Aosang Monosodium Glutamate Food Limited Company,Guangzhou 510280 ,China |
| Abstract: | Inconsistence of glutamic acid production with yield of glutamic acid to glucose often occurs in high biotin fermentation process because of limited fermentor dissolved oxygen coefficient. Glutamic acid fermentation process with two inoculations and additions of biotin were investigated to solve the inconsistence. Second inoculation and 3 μg/L biotin were added after cultivation for some time with 8μg/L biotin in the fermentor. Glutamic acid production reached 139.6g/L and yield of glutamic acid to glucose reached 62.80%. The single fermentor glutamic acid outcome increased by 15.78% than that of single inoculation and addition of 8.0μg/L biotin. |
| Keywords: | two inoculations repeated addition biotin glutamic acid dissolved oxygen |
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