烟草中游离氨基酸的自动分析仪测定

以茚三酮为显色剂(溶液流速:0.30mL/min),柠檬酸钠水溶液为缓冲液(流速:0.45/min),在柱温53℃、反应温度138℃及570nm和440nm检测波长下利用氨基酸自动分析仪测定了国产和进口18种白肋烟烟叶和11种混合型卷烟中的游离氨基酸含量。结果表明:①进口白肋烟烟叶的游离氨基酸总量明显高于国产烟叶;②进口白肋烟中的天冬氨酸、谷氨酸含量高于国产烟叶,而国产白肋烟中的脯氨酸含量高于进口烟叶;③进口混合型卷烟的游离氨基酸总量明显高于国产卷烟,尤其是天冬氨酸、白氨酸、酪氨酸和脯氨酸。

Determination of Free Amino Acids in Tobacco with Automatic Analyzer

The free amino acids in 18 kinds of burley tobacco and 11 brands of blended type cigarette were determined by automatic amino acid analyzer (AAAA). The working conditions of AAAA were: color reagent was ninhydrin solution (flow rate at 0.30mL/min), buffering solution was sodium citrate aqueous solution (flow rate at (0.45mL/min),)and detection wavelengths were 570nm and 440 nm. The results showed that: 1) the total free amino acid in imported burley tobacco was obviously higher than that in domestic tobacco; 2) asparaginic acid and glutamic acid in imported (tobacco) were higher than those in domestic tobacco,while the proline content in imported tobacco was lower than that in domestic tobacco; 3) the total free amino acid in imported blended type cigarette was significantly higher than that in domestic cigarette,especially for asparaginic acid,leucine,tyrosine and proline.