卵转铁蛋白对免疫低下小鼠Th1/Th2细胞平衡的影响

目的:研究卵转铁蛋白(OVT)对免疫低下模型小鼠的免疫平衡恢复作用。方法:将健康小鼠随机分为正常对照组、模型对照组及OVT高剂量组、中剂量组和低剂量组,每组10只。OVT高、中、低剂量组分别以10、1、0.1mg/mL的OVT溶液每天灌胃,灌胃量为20mL/(kg.d)(以体质量计)。模型组对照组和正常对照组灌胃生理盐水20mL/(kg.d)。连续灌胃15d,于第15天灌胃后注射环磷酰胺(正常对照组除外),继续灌胃至第19天(由模型决定)进行检测。测定血清中IFN-γ和IL-4含量、IFN-γ/IL-4比值以及脾脏和胸腺指数。结果:模型对照组小鼠血清IFN-γ的浓度和IFN-γ/IL-4比值与正常对照组相比明显降低(P<0.01);高剂量组小鼠血清IFN-γ的浓度和IFN-γ/IL-4比值显著高于模型对照组(P<0.01),与正常对照组比较,无显著性差异;高剂量组的脾脏指数和胸腺指数与模型对照组相比明显提高(P<0.01)。结论:OVT能修复由环磷酰胺所致免疫低下模型小鼠的体液和细胞免疫失衡,增强免疫低下小鼠的免疫功能。

Effects of Ovotransferrin on Th1/Th2 Cells Balance in Immunosuppressed Mice

Objective: To investigate the repairing effects of ovotransferrin(OVT) on immune imbalance of mice induced by cyclophosphamide.Methods: Fifty mice were randomly divided into five groups(ten animals per group): normal control group,model control group,and high dosage(10 mg/mL),middle dosage(1 mg/mL) and low dosage(0.1 mg/mL) OVT groups.Normal and model control groups were administered with normal saline.Each mouse in the OVT groups was administered with OVT of different concentrations at a dosage of 20 mL/(k...