小黄鱼抗氧化肽制备条件的响应面优化

目的：优化胰蛋白酶(PTN 6.0S)酶解小黄鱼制备抗氧化肽的条件。方法：以酶解产物对1,1-二苯基苦基苯肼 (DPPH)自由基的清除率为指标，通过单因素试验及响应曲面法优化酶解条件。结果：酶解条件在pH7.0、温度52℃、时间19h、底物质量浓度4.2g/100mL，酶与底物比6‰时，产物对DPPH自由基的清除率达到最大值(46.90±0.3)%。响应面分析表明，酶解时间对产物的抗氧化活性有显著影响；温度与酶与底物比、时间与底物质量浓度、酶与底物比与底物质量浓度在酶解工艺中存在复杂的交互作用。

Process Conditions Optimization for Antioxidant Peptide Preparation from Small Yellow Croaker by Response Surface Methodology

Objective: To optimize trypsin (PTN 6.0S) hydrolysis conditions of small yellow croaker for preparing antioxidant peptides. Methods: The optimization was performed using one-factor-at-a-time method and response surface methodology based on DPPH radical scavenging activity. Results: The optimal hydrolysis conditions were substrate concentration of 4.2 g/100 mL, enzyme-to-substrate ratio of 6‰, hydrolysis time of 19 h and hydrolysis temperature of 52 ℃. Maximum DPPH scavenging rate was obtained under the system of pH 7.0, reaching (46.90 ± 0.3)%. Response surface analysis showed that hydrolysis time had a significant effect on antioxidant activity of products and that the interactions between hydrolysis temperature and enzyme-to-substrate ratio, between hydrolysis time and substrate concentration, and between enzyme-to-substrate ratio and substrate concentration were complicated.