| 高效液相色谱法测定苋菜等有色样品的维生素C |
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| 引用本文： | 吴增茹,宋曙辉,武兴德,朱雨杰,刘玲.高效液相色谱法测定苋菜等有色样品的维生素C[J].现代仪器,1998(4). |
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| 作者姓名： | 吴增茹 宋曙辉 武兴德 朱雨杰 刘玲 |
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| 作者单位： | 国家蔬菜系统工程技术研究中心，国家蔬菜系统工程技术研究中心，国家蔬菜系统工程技术研究中心，国家蔬菜系统工程技术研究中心，国家蔬菜系统工程技术研究中心 北京 100081，北京 100081，北京 100081，北京 100081，北京 100081 |
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| 摘 要： | 本研究用HPLC法测定苋菜中的维生素C,色谱条件为Nova-pak C_(18)柱(3.9×300mm),0.1％草酸流动相,紫外检测器(254nm)。该方法不受植物色素及还原剂的影响,为测定有色蔬菜样品的理想方法。对61个苋菜品种进行取样测定,结果显示,苋菜维生素C的含量范围是34～81mg/100g鲜重,其中最高达81.37mg/100g鲜重。
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| 关 键 词： | 维生素C 苋菜 高效液相色谱法 |
High-performance Liquid Chromatographic Determination of Vitamin C in three colored Amaranth |
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| Wu Zengru Song Shuhui Wu Xingde Zhu Yujie Liu ling.High-performance Liquid Chromatographic Determination of Vitamin C in three colored Amaranth[J].Modern Instruments,1998(4). |
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| Authors: | Wu Zengru Song Shuhui Wu Xingde Zhu Yujie Liu ling |
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| Affiliation: | National Vegetable Research Center Beijing 100081 |
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| Abstract: | Determination of vitamin C in three colored Amaranth was studied by high-performance liquid chromatography (HPLC) with C18 column, 0.1% oxalic acid eluant and ultraviolet (UV) detector (254nm). The method is not influenced by plant pigments and reducers, so it is an ideal method when samples with pigments are determined. Vitamin C in 61 varieties of three colored Amaranth were determined. The range of vitamin C content was 34~81mg/100g fresh weight. The highest was 81. 37mg/100g fresh weight.. |
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| Keywords: | vitamin C three colored Amaranth HPLC |
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