高效液相色谱法检测蔬菜中的吡虫啉残留

本文建立了一种果蔬中吡虫啉农药残留的高效液相色谱检测方法。样品经乙腈提取、盐析、LC-NH2小柱净化,采用高效液相色谱仪-紫外检测器进行测定,色谱柱:Waters Symmetry Shield C18(4.6×250 mm,5μm);柱温:30.0℃;检测波长:275 nm;流动相:甲醇:水:乙腈=55:25:20(体积比);流速:0.8 mL/min;进样量:10.0μL。吡虫啉在0.1 mg/L至1.0 mg/L的添加范围内线性良好,线性关系系数r为0.9925;添加水平为0.05~0.20 mg/kg时,西红柿样品的平均回收率为95.4%~106.8%,相对标准偏差为2.66%~7.99%。结果表明该方法具有快速、灵敏、准确等优点,适合蔬菜中吡虫啉农药残留的测定。

Determination of Imidaeloprid Pesticide Residue in Fresh Vegetables by HPLC

A novel high performance liquid chromatography(HPLC) method was developed for the analysis of imidaeloprid pesticide residue in fresh vegetables.Imidaeloprid was extracted from the samples by acetonitrile,purified with LC-NH2 column and determined by high performance liquid chromatography with UV detection.Symmetry Shield? C18 column(4.6×250 mm,5 μm) using the mixture of methanol,water and acetonitrile(55:25:20) as the mobile phase.The column temperature,flow rate,detection wavelength and sample volume were 30.0 ℃,0.8 mL/min,275 nm and 10.0 μL,respectively.Within the linear range of 0.1~1.0 mg/L,the correlation coefficient,the recoveries of imidaeloprid in tomato and the relative standard deviations(RSD) were 0.9925,95.4%~106.8 % and 2.66%~7.99%,respectively.The method was simple,effective and accurate.It can be applied to determinate imidaeloprid pesticide residue in fresh vegetables.