| 微波超声波联用萃取白藜芦醇及其苷的HPLC测定 |
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| 引用本文： | 马密霞,秦宁,闵清,胡文祥.微波超声波联用萃取白藜芦醇及其苷的HPLC测定[J].化工学报,2019,70(Z1):124-129. |
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| 作者姓名： | 马密霞 秦宁 闵清 胡文祥 |
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| 作者单位： | 1. 武汉工程大学化学与环境工程学院, 湖北 武汉 4300732. 北京联合大学智慧城市学院, 北京 1001013. 湖北科技学院药学院, 湖北 咸宁 4371004. 北京神剑天军医学科学院京东祥鹄微波化学联合实验室, 北京 101601 |
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| 基金项目： | 北京市创新基金项目(Z080104024508111) |
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| 摘 要： | 建立了花生芽和葡萄籽微波超声波联用提取白黎芦醇及其苷的HPLC测定方法。采用高效液相色谱进行测定,色谱柱为迪马C18, 流动相为乙腈-水溶液,梯度洗脱,流速1.0 ml/min,柱温为30℃,检测波长为306 nm,进样量为20 μl。白黎芦醇在0.343～15.15×103 μg/ml范围内呈良好的线性关系,相关系数R2 = 0.9967;白藜芦醇苷在110.712～1774.23×103 μg /ml范围内线性关系良好,相关系数R2 = 0.9993。该方法检测快速、简便、重现性好,适用于花生芽和葡萄籽中白藜芦醇及其苷的定量分析。
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| 关 键 词： | 微波超声波 萃取 白藜芦醇 药物 色谱 浸取 |
| 收稿时间： | 2018-12-17 |
| 修稿时间： | 2019-03-02 |
Resveratrol and polydatin by microwave-ultrasound extraction and determination by HPLC |
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| Mixia MA,Ning QIN,Qing MIN,Wenxiang HU.Resveratrol and polydatin by microwave-ultrasound extraction and determination by HPLC[J].Journal of Chemical Industry and Engineering(China),2019,70(Z1):124-129. |
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| Authors: | Mixia MA Ning QIN Qing MIN Wenxiang HU |
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| Affiliation: | 1. School of Chemical and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430073, Hubei, China2. Smart City College, Beijing Union University, Beijing 100101, China3. School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, Hubei, China4. Jingdong Xianghu Microwave Chemistry Union Laboratory, Beijing Excalibur Space Military Academy of Medical Sciences, Beijing 101601, China |
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| Abstract: | Objective to establish a method for the determination of resveratrol and polydatin in peanut bud and grape pip extract. High performance liquid chromatography (HPLC) was used. The chromatographic column was Di Ma C18, the mobile phase was acetonitrile water solution, gradient elution, flow rate 1 ml/min, column temperature 30℃, 306 nm and 20 μl. The linear range of resveratol showed good linear relationship 0.343－15.15×103μg /ml, R2 = 0.9967. The linear range of polydatin showed good linear relationship 110.712－1774.23×103 μg/ml, R2 = 0.9993. The method is rapid, simple and reproducible, and is suitable for quantitative analysis of resveratrol in peanut sprouts and grape seeds. |
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| Keywords: | microwave ultrasonic wave extraction resveratrol pharmaceuticals chromatography leaching |
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