| 乙酰胆碱酯酶在有机磷和氨基甲酸酯类农药快速检测中的应用 |
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| 引用本文： | 李昕,王忠斌,张燕,王硕. 乙酰胆碱酯酶在有机磷和氨基甲酸酯类农药快速检测中的应用[J]. 食品研究与开发, 2007, 28(3): 127-129 |
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| 作者姓名： | 李昕 王忠斌 张燕 王硕 |
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| 作者单位： | 天津市食品营养与安全重点实验室,天津科技大学食品工程与生物技术学院,天津,300457 |
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| 摘 要： | 为满足食品中有机磷和氨基甲酸酯类农药的快速检测，研究采用酶抑制显色方法，应用自制的乙酰胆碱酯酶制成农药快速检测试剂盒检测有机磷和氨基甲酸酯类农药，检测灵敏度在0．001mg／kg-0．1mg／kg范围。试剂盒为现场检测这两类农药提供了简便而快捷的方法。
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| 关 键 词： | 乙酰胆碱酯酶 有机磷 氨基甲酸酯 快速检测 农药残留 |
| 修稿时间： | 2006-10-31 |
THE RAPID DETECTION OF ORGANOPHOSPHORUS AND CARBAMATE PESTICIDES BY ACETYLCHOLINESTERASE(ACHE)FROM MUSCA DOMESTICA |
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| LI Xin,WANG Zhong-bin,ZHANG Yan,WANG Shuo. THE RAPID DETECTION OF ORGANOPHOSPHORUS AND CARBAMATE PESTICIDES BY ACETYLCHOLINESTERASE(ACHE)FROM MUSCA DOMESTICA[J]. Food Research and Developent, 2007, 28(3): 127-129 |
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| Authors: | LI Xin WANG Zhong-bin ZHANG Yan WANG Shuo |
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| Affiliation: | Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China |
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| Abstract: | Purified acetylcholinesterase (AChE) was made from Musca domestica by Procainamide-Based affinity chromatography. Organophosphorus and carbamate pesticides could be rapid detected by the color developed during enzymatic reaction and the detecting sensitivity is 0.001 mg/kg~0.1 mg/kg. The method is convenient and time-saving. |
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| Keywords: | acetylcholinesterase organophosphorus pesticide carbamate pesticide rapid detection pesticide residue |
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