和厚朴酚分子印迹聚合物微球的制备及表征

采用单步溶胀聚合法制备了和厚朴酚分子印迹聚合物微球(MIPMs)。Scatchard分析表明分子印迹聚合物微球在识别和厚朴酚分子的过程中存在两类结合位点,其高亲和位点的解离常数为KD1=0.13 mmol/L,最大表观结合量Qmax=45.21μmol/g。静态吸附试验证明MIPMs对模板分子具有较强的特异吸附能力,以厚朴酚为竞争底物,其分离因子达1.85。

Preparation and Characterization of Honokiol Molecularly Imprinted Polymeric Microspheres

The honokiol molecularly imprinted polymeric microspheres were successfully prepared in aqueous system by a one-step swelling and polymerization method.Scatchard analysis showed that there were two classes of binding sites formed in the imprinted microspheres.The equilibrium dissociation constant of binding sites with the higher affinity was estimated to be 0.13 mmol/L,and the density of the calculated maximum binding sites was 45.21 μmol/g.The resultant honokiol imprinted microspheres showed the predominate honokiol recognition coefficient,which was 1.85 times higher than that for the magnolol recognition.