高效液相色谱-示差折光检测器测定柴油中多环芳烃

本文开展了柴油中多环芳烃(PAHs)的高效液相色谱(HPLC)分析方法的研究。氨基柱做固定相,正庚烷为流动相,采用示差折光检测器进行定性和定量分析。优化了柱流量和反冲洗时间,当柴油样品中双环芳烃(DAHs)加标浓度水平分别为0.8、2.0、4.0g/100mL,三环(及三环以上)芳烃(T+AHs)加标浓度水平分别为0.08、0.20、0.40g/100mL时,加标回收率分别为94%～108%和92%～106%,相对标准偏差分别为0.72%～3.18%及1.14～5.46%。该分析方法已成功地应用于柴油中PAHs的分析,其结果令人满意。

Determination of PAHs in Diesel by High Performance Liquid Chromatography with Refractive Index Detector

An analytical method was developed for the determination of PAHs in diesel by HPLC-RID.The Zorbax NH3 columns was as immobile phase,and the mobile phase was heptane. Samples were diluted by heptane and then determined by HPLC-RID.Finally,the flow rate of the mobile phase and the backflush were optimized. The recovery studies were performed at 0.8、2.0、4.0g/100mL fortification levels for DAHs, and 0.08、0.20、0.40 g/100mL for T＋AHs, the recoveries ranged from 94% ～108% with a relative standard deviation between 0.72% and 3.18% for DAHs,and 92% ～106% with a relative standard deviation between 1.14% and 5.46% for T＋AHs。