| 胶原蛋白酶产生菌的筛选及初步鉴定 |
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| 引用本文： | 邓加聪,郑虹,陈丽琴.胶原蛋白酶产生菌的筛选及初步鉴定[J].中国酿造,2013,32(4):78. |
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| 作者姓名： | 邓加聪 郑虹 陈丽琴 |
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| 作者单位： | 福建师范大学福清分校生物与化学工程系,福建福清,350300 |
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| 摘 要： | 从养殖场附近淤泥中采样,分离得到20株胶原蛋白水解活性的菌株.经活性平板初筛、摇床复筛及酶活测定,筛选得到1株胶原蛋白酶活性为28.70U/mL的菌株.通过对该菌株16S rDNA的blast检索及序列分析表明,该菌株与蜡状芽孢杆菌属有99％以上的同源性,结合生理生化实验结果,经形态观察、生化试验,初步鉴定该菌株为蜡状芽孢杆菌.
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| 关 键 词： | 胶原蛋白 胶原蛋白酶 菌株筛选 |
Screening and preliminary identification of collagenase-producing bacteria |
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| Abstract: | Twenty strains with collagen hydrolyzation activity were isolated from the soil of the pig mark. The strain with collagenase activity about 28.70U/ml was obtained by primary plate screening, secondary incubator screening and enzyme activity assay. Blast analysis showed that the 16S rDNA of the strain was 99% similarity with Bacillus cereus . The result including biochemical tests indicated that the stain MAP-K5 was belonged to Bacillus cereus. |
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| Keywords: | collagen collagenase strain screening |
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