肝硬化对大鼠心肌肌球蛋白重链表达的影响

目的:研究肝硬化对大鼠心肌肌球蛋白重链α、β亚型表达的影响。方法:取清洁级SD大鼠30只,随机分为两组:对照组和肝硬化组。记录各组大鼠的心脏系数,测定各组心脏血流动力学指标,RT-PCR法检测各组心肌肌球蛋白重链α、β亚型表达的变化。结果:与对照组相比,肝硬化组心脏系数增大,心脏LVSP, +dp/dt_max,-dp/dt_max降低,RT-PCR法检测心肌α-MHC基因表达降低、β-MHC基因表达升高。结论:肝硬化影响心肌的收缩功能,同时刺激心肌肌球蛋白重链基因表达发生变化,提示其可能参与了肝硬化心肌病的发生和发展。

Effects of liver cirrhosis on the expression of myosin heavy chain in rat myocardium

AIM: To study the effects of liver cirrhosis on the expressions of myosin heavy chain (MHC) isoforms in rat myocardium. METHODS: Thirty male Sprague-Dawley rats were randomly divided into control group and liver cirrhosis group. The heart weight to body weight ratio (H/B) were recorded. The ventricular hemodynamical parameters in vitro were determined. α-MHC mRNA and β-MHC mRNA expression of myocardium was evaluated by RT-PCR.RESULTS: In contrast to normal rat,H/B were increased , left ventricular systolic pressure LVSP, maximal rise/fall rate of left ventricular pressure ±dp/dt_max were decreased in model rat. The expression of α-MHC mRNA was decreased,and the expression of β-MHC mRNA was in the opposite.CONCLUSION: Liver cirrhosis affects the function of myocardial and stimulates the expressional change of myosin heavy chain gene in rat myocardium, which is related with the occurrence and development of cirrhotic cardiomyopathy.