响应面法优化芫花总黄酮的提取工艺

以乙醇为溶剂,采用回流提取的方法,建立芫花中总黄酮类物质的最佳提取工艺。在单因素实验的基础上,运用四因素三水平的Box-Behnken中心组合试验设计原理进行实验设计,建立提取温度、提取时间、乙醇浓度、料液比与芫花总黄酮得率之间的数学模型。该模型的响应面分析结果表明,所建立的回归方程极显著,拟合性好。在总的作用因素中,平方项对总黄酮的得率的影响最为显著,其次为一次项,交互项的影响相对较小。在回流提取条件下,乙醇浓度对总黄酮得率的影响最大,其次是料液比,提取时间和提取温度影响较小。最终得出芫花总黄酮的最佳提取工艺为:提取温度73℃、提取时间1h、乙醇浓度78%、料液比料液比1∶12(g/mL),在此条件下,芫花总黄酮得率为2.239%。

Optimization of reflux extraction of total flavonoids from genkwa flos using response surface analysis

An optimal reflux extraction of total flavonoids from genkwa flos was established.Based on the single factor analysis,a 4-factor,3-level Box-Behnken experimental design was applied to evaluate the effects of the four factors,including extraction temperature,extraction time,ethanol concentration and ratio of material to liquid.The interactions of each factor on the extraction yield were investigated with response surface analysis(RSA).Results showed that the developed mathematical model for total flavonoids yield fit the actual experiment very well.A significant effect on total flavonoids yield was observed in quadratic and linear terms.However,the interactions among the above parameters were not significant.Ethanol concentration had the most significant effect,ratio of material to liquid was the second place and extraction time,extraction temperature had a little effect.The final optimal extracting conditions were as follows:extraction temperature was 73 oC,extraction time was 1 h,ethanol concentration was 78% and ratio of material to liquid was 1∶12(g/mL).Under the above conditions the extraction yield was 2.239%.