肝癌小鼠血清荧光谱峰位蓝移及分子振动机理研究

通过多峰高斯拟合方法,对接种了肝癌小鼠生长 5~15天期间的血清荧光光谱进行系统研究,结 果表明,随着肿瘤的生长,荧光谱曲线及其高斯基元函数的峰值位置都有向短波方向移动的 趋势。基于分 子振动理论的分析认为,是肿瘤生长过程中,血清中部分发光分子的含量增加,其分子趋于 彼此靠近,分 子间的相互作用增强,分子中原子间化学键趋于伸长,其基态的振动能级趋于降低,若第一 电子激发态的 最低振动能级未发生变化,则基态与第一电子激发态最低振动能级之间的能量差趋于增大, 导致相关荧光 谱曲线的峰值位置趋于蓝移。本文的研究结果表明,对血清荧光光谱进行非线性多峰拟合及 分子振动理论 分析,有助于从分子层面揭示血清中荧光物质变化的深层次原因。

The study of the peak position blue shift and molecular vibration mechanism of serum fluorescence spectra in mice with liver cancer

By using the method of multi-peak Gaussian fitting,the fluorescence spe ctra of serum from 5-15days of liver cancer mice were studied,and the results show that with the growth of tumor,the peak position of the fluorescence composing line and its Gaussian func tion move to short wave direction. Based on the theory of molecular vibration,it is believed that in the course of tumor growth,the content of some luminous molecules in the serum increases, the molecules tend to be close to each other,the interaction between the molecules is enhanced,the chem ical bond between the atoms tends to elongate,and the vibrational energy level of the atomic state is reduced. If the lowest vibrational level of the first electron excitation state is not changed,the ene rgy difference between the ground state and the lowest vibrational level of the first electron excited stat es tends to increase,resulting in the peak position of the correlated fluorescence spectral curve becoming blue shift. The results of this study show that the nonlinear multi-peak fitting and molecular vibration analys is of serum fluorescence spectra are helpful to reveal the deep-seated causes of the changes of fluoresc ent substances in serum from the molecular level.