| Abstract: | ![]()
Modified methods for the determination of bound hydroxyproline in blood are presented. The fractionated and hydrolyzed samples are separated from interfering material by ion exchange chromatography. Internal standards are used to correct for recovery. After a several fold concentration of samples it is possible to work with reduced blood volumes. Up to 6.12 mumol/l of free and peptide-bound and 11.5 mumol/l of protein-bound hydroxyproline are detectable. Using the described methods hydroxyproline was determined in a pooled human plasma and in the sera of rats. |
