| 鸡蛋溶菌酶生产过程活力动态监控 |
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| 引用本文： | 石军英,郭雄军,陈劲春.鸡蛋溶菌酶生产过程活力动态监控[J].食品科技,2008,33(11). |
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| 作者姓名： | 石军英 郭雄军 陈劲春 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京,100029 |
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| 摘 要： | 通过对传统的比浊法中菌体的培养条件以及测定方法的改进,确定菌液的接种量为0.5mL,培养时间是24.5h,菌液的OD值调节到0.8,以及待测样品稀释倍数。成功地克服了菌在测定过程中不稳定、重复性差的问题,准确地测定了溶菌酶生产过程中各个步骤的样品活力,体现了生产过程的基本变化趋势,为溶菌酶生产过程提供了可靠的检测依据。
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| 关 键 词： | 溶菌酶 跟踪监测 溶壁微球菌 重复性差 |
Dynamic monitoring of egg Lysozyme bioactivity during preparation in an industrial scale |
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| SHI Jun-ying,GUO Xiong-jun,CHEN Jin-chun.Dynamic monitoring of egg Lysozyme bioactivity during preparation in an industrial scale[J].Food Science and Technology,2008,33(11). |
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| Authors: | SHI Jun-ying GUO Xiong-jun CHEN Jin-chun |
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| Abstract: | In this report, based on the improvement of cell culture conditions and the methods, the instability and poor reproducibility of the cell existing in traditional turbidimetrlc are overcomed successfully. The vitalities of the samples in the various steps of the production process are determinated accurately to show the basic trend of the production process, and provide a reliable detection basis for production process. |
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| Keywords: | Lysozyme tracking dissolved wall micrococcus poor repeatability |
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