| PEG/（NH4）2SO4双水相萃取分离茶氨酸的研究 |
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| 引用本文： | 王艳,彭奇均.PEG/（NH4）2SO4双水相萃取分离茶氨酸的研究[J].应用化工,2011,40(7):1186-1191,1208. |
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| 作者姓名： | 王艳 彭奇均 |
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| 作者单位： | 江南大学,化学与材料工程学院,江苏,无锡,214122 |
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| 基金项目： | 中央高校基本科研业务费专项资金资助 |
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| 摘 要： | 采用聚乙二醇(PEG)/(NH4)2SO4双水相体系萃取分离生产茶多酚所得废液中的茶氨酸,考察了PEG分子量与含量、硫酸铵含量、pH、温度、加盐(KCl、KBr、KI)、茶氨酸含量对双水相及萃取分离茶氨酸的影响。结果表明,PEG/(NH4)2SO4双水相萃取分离茶氨酸的适宜条件是:PEG平均分子量为4 000,质量分数为10%,硫酸铵质量分数为15%,pH约为6,30℃。在此条件下,茶氨酸的分配系数K1=0.16,蛋白质的分配系数K2=0.28,糖类的分配系数K3=9.8,茶氨酸在下相的萃取率为89.5%,可以将茶多酚废液中的茶氨酸与糖类及其他有颜色的杂质分开。
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| 关 键 词： | 双水相 茶氨酸 分配系数 萃取率 |
Study on theanine extraction separation by PEG/(NH4)2SO4aqueous two-phase system |
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| WANG Yan,PENG Qi-jun.Study on theanine extraction separation by PEG/(NH4)2SO4aqueous two-phase system[J].Applied chemical industry,2011,40(7):1186-1191,1208. |
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| Authors: | WANG Yan PENG Qi-jun |
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| Affiliation: | WANG Yan,PENG Qi-jun (College of Chemical and Materials Engineering,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | The polyethylene glycol(PEG)/(NH4)2SO4 aqueous two-phase system was used to extract the theanine from the tea polyphenol waste liguid.Effect of the molecular weight and concentration of PEG,concentration of(NH4)2SO4,pH,temperature,salt(KCl,KBr,KI),concentration of the theanine on the aqueous two-phase and extraction of theanine was studied.The results showed that the appropriate conditions were as follows:the average molecular weight of PEG was 4 000,the quality percentage of PEG was 10%,the quality percent... |
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| Keywords: | aqueous two-phase theanine distribution coefficient rate of extraction |
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