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Letter to the Editor
Authors:Werner Villiger
Abstract:A double-disector method for obtaining estimates of the number of particles inside other particles, illustrated by an estimation of the average cell number in a glomerulus, has been developed. The method is suitable for paraffin-embedded tissue because it does not require knowledge about the section thickness. Most importantly, the estimate is absolutely unaffected by tissue shrinkage. The average number of cells in a human glomerulus is 2850 whereas in rats it is 580.
Keywords:Disector  double disector  particle number  renal glomeruli  sampling  section thickness  stereology
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