Recovery of eicosapentaenoic acid from fungal mycelia by solvent extraction |
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Authors: | Dennis J O’Brien Gerard E Senske |
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Affiliation: | (1) USDA, ARS, ERRC, 600 East Mermaid Lane, 19118 Philadelphia, Pennsylvania |
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Abstract: | Utilization of lipids containing eicosapentaenoic acid (EPA) produced by microorganisms requires processes for their efficient
recovery from microbial cells. Recovery of EPA from mycelia of the fungusPythium irregulare by solvent extraction with hexane-isopropanol (HIP) in a pilot-plant colloid mill was investigated. Extraction efficiencies
of 96% for lipid and EPA were achieved with a 3∶2 (vol/vol) HIP mixture by milling wet, filtered mycelia for 5 min at a solvent/dry
solids ratio of 100 L/kg. The process yielded a crude extract that contained up to 96% lipid and an EPA content as high as
24% (with no selectivity for EPA). |
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Keywords: | Colloid mill fatty acid hexane isopropanol lipid polyunsaturated fatty acid Pythium irregulare |
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