纤维素酶辅助超声法提取葛根多糖及其DPPH自由基清除能力

本文以柴葛根为原料,以纤维素酶辅助超声法提取葛根多糖的工艺并对其DPPH自由基清除能力进行研究。在单因素研究结果基础上,确定纤维素酶的用量、超声功率、超声时间和超声温度四因素三水平Box-Benhnken组合试验,优化多糖得率条件。结果表明:在纤维素酶用量6.0%,超声功率280 W,超声时间为52 min,超声温度65℃条件下,多糖得率为4.93%±0.02%,与预测值的误差在2%之内。酶辅助超声法提取的葛根多糖对DPPH清除自由基能力IC₅₀值为954.97 μg/mL,热回流法的IC₅₀值为1379.60 μg/mL,维生素C为131.07 μg/mL。因此,纤维素酶辅助超声法提取葛根多糖具有较强清除DPPH自由基活性,为葛根多糖的开发利用的IC₅₀值提供依据。

Cellulase Assisted Ultrasound Extraction of Pueraria Polysaccharides and Its DPPH Free Radical Scavenging Ability

In this paper,taking the Pueraria lobata roots as raw material,the methods of cellulase auxiliary ultrasound Pueraria lobata polysaccharides and the DPPH free radical scavenging ability of Pueraria lobata polysacharides was also analyzed. On the basis of single factor experiments,three levels of the four factors(cellulose dosage,ultrasonic power,ultrasonic time,ultrasonic temperature)were selected for Box-Benhnken design to optimize the polysaccharide yield rate conditions. The result showed that,cellulase dosage was 6.0%,ultrasonic power was 280 W,ultrasonic time was 52 min,ultrasonic temperature was 65℃,the actual polysaccharide yield rate was 4.93%±0.02%. Compared with the predicted value,the error was within 2%. And the DPPH free radical scavenging capacity of the polysaccharides extracted by enzyme-assisted ultrasonic extraction had IC₅₀ value of 954.97 μg/mL,IC₅₀ value of 1379.60 μg/mL by thermal reflux method,and IC₅₀ value 131.07 μg/mL by V_C. Pueraria polysaccharide had strong activity of scavenging DPPH free radical by cellulase assisted ultrasound extraction. It would provide the basis for the development and utilization of radix Pueraria lobate polysaccharides.