玉米黄素对衣霉素诱导的SH-SY5Y细胞损伤的保护作用

研究玉米黄素对衣霉素诱导损伤的神经母细胞瘤细胞（SH-SY5Y）的保护作用。实验分为空白组、损伤组（模型组）、2、5、10 μmol/L玉米黄素保护组。用衣霉素（tunicamycin，TM）建立SH-SY5Y细胞损伤模型，噻唑蓝测定细胞存活率，试剂盒法测定培养液中的乳酸脱氢酶（lactate dehydrogenase，LDH）水平。结果显示：TM质量浓度在2 μg/mL以上时，可显著降低SH-SY5Y细胞活性（P＜0.01）；与TM损伤组相比，2～10 μmol/L玉米黄素预处理可显著提高细胞存活率（P＜0.05），降低LDH的释放率，先用TM处理30 min后再加2、5、10 μmol/L的玉米黄素处理24 h的玉米黄素后处理组结果相同。因此，TM通过诱导内质网应激可引起细胞损伤，玉米黄素能够减轻该损伤作用，并且在一定程度上可逆转TM引起的损伤。

Protective Effect of Zeaxanthin on SH-SY5Y Cell Injury Induced by Tunicamycin

This study was designed to evaluate the protective effect of zeaxanthin on SH-SY5Y cell injury induced by tunicamycin (TM) in vitro. The experiment was divided into control group, model group and treatment groups with different doses of zeaxanthin. Cell viability was measured by methyl thiazolyl tetrazolium (MTT) assay, and lactate dehydrogenase (LDH) in cells and cell culture were determined by a commercial kit. The results showed that TM at concentrations over 2 μg/mL could significantly decrease cell viability (P < 0.01). In contrast to the model group, the pretreatment of zeaxanthin in the concentration range of 2–10 μmol/L could increase cell viability (P < 0.05) and suppress TM-induced LDH release in SH-SY5Y cells. The same results were obtained with the reverse sequence. TM caused cell injury by inducing endoplasmic reticulum stress. This effect was reduced or even abrogated by zeaxanthin.