畜禽肉中抗生素残留检测技术研究进展

畜禽肉中抗生素残留问题已成为各方广泛关注的突出问题。畜禽肉中的抗生素残留可能来自用于饲料和添加剂中,或者是用于兽医临诊,或者是来自于在休药期违法使用。畜禽肉中的抗生素残留可能造成的危害包括对人体的危害、对生态环境的危害和对养殖业的危害。因此开发畜禽肉中抗生素残留的快速、高效、简便的前处理和检测方法成为迫切的需求。本文综述了畜禽肉中抗生素的不同检测和前处理技术及其进展。目前,用于畜禽肉中抗生素残留的检测方法主要有理化检测法(包含薄层色谱法和毛细管电泳法等)、生物检测法(包含微生物检测法、免疫分析法和生物传感器法等)、高效液相色谱法和液相色谱-质谱/质谱法等。对畜禽肉中抗生素残留进行前处理的新技术有加压液体萃取、微波辅助萃取、基质固相分散、分散固相萃取、固相微萃取等。

Progress of the detection technology of antibiotic residues in livestock and poultry meat

The problem of antibiotic residues in livestock and poultry has become a prominent issue of wide concern. Antibiotic residues in livestock and poultry meat may come from use in feeds and additives, or in veterinary clinics, or from illegal use during the drug holiday. The possible harms caused by antibiotic residues in livestock and poultry meat include harm to the human body, damage to the ecological environment and harm to the breeding industry. Therefore, it is urgent to develop a rapid, efficient and simple pretreatment and detection method for antibiotic residues in livestock and poultry meat. This paper reviewed the different detection and pretreatment techniques and advances of antibiotics in livestock and poultry meat. At present, the detection methods for antibiotic residues in livestock and poultry meat mainly included physical and chemical detection methods (including thin layer chromatography and capillary electrophoresis), biological detection methods (including microbial detection methods, immunoassays, biosensor methods, etc.). New technologies for pretreatment of antibiotic residues in livestock and poultry meat included pressurized liquid extraction, microwave assisted extraction, matrix solid phase dispersion, dispersed solid phase extraction, and solid phase microextraction, etc.