模拟胃肠道消化过程中迷迭香提取物总酚及抗氧化活性变化

本文研究了迷迭香提取物在模拟胃肠道消化过程中总酚含量及抗氧化活性的变化规律,并对其总酚含量变化与抗氧化活性进行相关性分析。结果表明,迷迭香提取物消化产物中总酚含量随模拟胃消化时间的延长而升高,而在模拟肠消化过程中显著降低(P<0.05)。经模拟胃消化180 min后,总酚含量显著升高了17.88%(P<0.05),而经模拟肠消化120 min后,总酚含量显著降低了19.64%(P<0.05)。经模拟胃消化180 min,消化产物对DPPH·和超氧阴离子自由基的清除能力分别显著提高了20.36%和22.07%(P<0.05),铁还原能力(Ferric ion reducing antioxidant power,FRAP)显著提高了11.76%(P<0.05)。而经模拟肠消化120 min后,消化产物对DPPH·和超氧阴离子自由基的清除能力分别显著降低了74.08%和50.00%(P<0.05),FRAP显著降低了52.13%(P<0.05)。此外,迷迭香提取物消化产物的抗氧化活性与其总酚含量之间存在良好的正相关性。以上结果表明,模拟胃肠道消化过程显著影响迷迭香多酚的含量和抗氧化活性。

Changes in Total Polyphenols and Antioxidant Activity in Rosemary Extract during Simulated Gastrointestinal Digestion

The changes in total polyphenols contents and antioxidant activity of rosemary extract during simulated gastrointestinal digestion process were investigated in this work,and the relationship between the contents of total polyphenols and the antioxidant activity was verified. The results showed that the total polyphenols contents in the digestive products of rosemary extracts increased significantly during the in vitro gastric digestion,while the simulated intestinal digestion resulted in the substantial loss of phenolic compounds(P<0.05). After the simulated gastric digestion for 180 min,the total polyphenols contents in the digestive products increased by 17.88%(P<0.05). However,the total polyphenols contents decreased by 19.64% after the simulated intestinal digestion for 120 min(P<0.05). Following the simulated gastric digestion for 180 min,the scavenging activities of the digestive products against DPPH radical and superoxide radical increased by 20.36% and 22.07%(P<0.05),respectively. Moreover,the ferric ion reducing antioxidant power(FRAP)increased by 11.76%(P<0.05). However,following the simulated intestinal digestion for 120 min,the scavenging activities of the digestive products against DPPH radical and superoxide radical were reduced by 74.08% and 50.00%(P<0.05),respectively,and the FRAP value decreased by 52.13%(P<0.05). In addition,there was a positive relationship between the antioxidant activity potential and the total phenolic of the digestive products. In summary,the simulated gastrointestinal digestion results in significant influences on the total polyphenols contents and antioxidant activity of rosemary extract.