| 25-羟基维生素D检测方法研究进展 |
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| 引用本文： | 曾伟杰,谢 洁,龚晓云,翟 睿,黄泽建,刘梅英,徐锐峰,江 游,戴新华,方 向,时国庆.25-羟基维生素D检测方法研究进展[J].食品安全质量检测技术,2019,10(11):3419-3429. |
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| 作者姓名： | 曾伟杰 谢 洁 龚晓云 翟 睿 黄泽建 刘梅英 徐锐峰 江 游 戴新华 方 向 时国庆 |
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| 作者单位： | 北京科技大学化学与生物工程学院;中国计量科学研究院前沿计量科学中心质谱仪器工程技术研究中心 |
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| 基金项目： | 国家重大科学仪器设备开发项目(2018YFF0109504)、中国计量科学研究院基本科研业务费项目(AKY1934, AKYCX1904, AKYKF1809) |
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| 摘 要： | 维生素D(vitamin D, VD)是人体内一种重要的调节因子,除参与人体内钙磷代谢,其在高血压、心血管疾病、肿瘤及糖尿病等疾病中也扮演着重要角色。25羟基维生素D(25-hydroxy vitamin D, 25(OH)D)是VD在人体内的主要存在形式,性质稳定(半衰期2周),其水平能充分反映食物摄入和自身合成VD总量,在精准药物治疗与营养膳食理念方面具有重要意义,因此,需要高准确测定25(OH)D浓度水平。本文综述了25(OH)D的前处理方法和检测方法,主要包括放射免疫法、酶联免疫吸附法、化学发光免疫测定法、高效液相色谱法和液相色谱-串联质谱法,并展望了其发展趋势。
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| 关 键 词： | 25-羟基维生素D 合成及代谢途径 前处理方法 检测方法 |
| 收稿时间： | 2019/4/30 0:00:00 |
| 修稿时间： | 2019/5/14 0:00:00 |
Research progress on determination methods of 25-hydroxy vitamin D |
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| ZENG Wei-Jie,XIE Jie,GONG Xiao-Yun,ZHAI Rui,HUANG Ze-Jian,LIU Mei-Ying,XU Rui-Feng,JIANG You,DAI Xin-Hu,FANG Xiang and SHI Guo-Qing.Research progress on determination methods of 25-hydroxy vitamin D[J].Food Safety and Quality Detection Technology,2019,10(11):3419-3429. |
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| Authors: | ZENG Wei-Jie XIE Jie GONG Xiao-Yun ZHAI Rui HUANG Ze-Jian LIU Mei-Ying XU Rui-Feng JIANG You DAI Xin-Hu FANG Xiang and SHI Guo-Qing |
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| Affiliation: | School of Chemistry and Biological Engineering, University of Science and Technology Beijing,Division of Chemical Metrology and Analytical Science,Mass Spectrometry Engineering Technology Research Center, Center for Advanced Measurement Science, National Institute of Metrology,Mass Spectrometry Engineering Technology Research Center, Center for Advanced Measurement Science, National Institute of Metrology,Mass Spectrometry Engineering Technology Research Center, Center for Advanced Measurement Science, National Institute of Metrology,Mass Spectrometry Engineering Technology Research Center, Center for Advanced Measurement Science, National Institute of Metrology,Mass Spectrometry Engineering Technology Research Center, Center for Advanced Measurement Science, National Institute of Metrology,Mass Spectrometry Engineering Technology Research Center, Center for Advanced Measurement Science, National Institute of Metrology,Mass Spectrometry Engineering Technology Research Center, Center for Advanced Measurement Science, National Institute of Metrology,Mass Spectrometry Engineering Technology Research Center, Center for Advanced Measurement Science, National Institute of Metrology and School of Chemistry and Biological Engineering, University of Science and Technology Beijing |
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| Abstract: | Vitamin D (VD) is an important regulator in the human body. In addition to participating in calcium and phosphorus metabolism in humans, VD also plays an important role in diseases such as hypertension, cardiovascular disease, cancer and diabetes. 25-hydroxy vitamin D (25(OH)D) is the main form of VD in the human body, and the properties are stable (half-life of 2 weeks). The level can fully reflect the food intake and the total amount of VD synthesized by itself, which is of great importance in the context of precision drug therapy and nutritional diets, and therefore requires a high level of accurate determination of 25(OH)D levels. This paper summarized the pretreatment methods and detection methods of 25(OH)D, including radioimmunoassay, enzyme-linked immunosorbent assay, chemiluminescence immunoassay, high performance liquid chromatography and liquid chromatography-tandem mass spectrometry, and looked forward to the development trends. |
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| Keywords: | 25-hydroxy vitamin D synthesis and metabolic pathways pretreatment detection methods |
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