酒花浸膏关键性香气成分分析

采用固相微萃取(solid-phase microextraction,SPME)与溶剂辅助蒸发萃取(solvent-assisted evaporative extraction,SAFE)法结合气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)联用技术分析酒花浸膏中的香气成分,同时采用气相色谱-嗅闻仪(gas chromatography-olfactory,GC-O)结合香气提取稀释分析(aroma extraction dilution analysis,AEDA)方法和香气活性值(odor activity value,OAV),最终确定酒花浸膏的关键性香气成分。结果显示,采用2种萃取方法结合GC-MS在酒花浸膏中共鉴定出111种香气成分;经GC-O分析共鉴定出27种香气活性化合物,其中异丁酸乙酯、丁二酸二乙酯、2-甲基丁酸含量最高,结合风味稀释因子和OAV分析共同确认酒花浸膏的关键香气成分为异丁酸乙酯、2-甲基丁酸、异己酸乙酯、辛酸乙酯、癸酸乙酯等化合物。

Identification of the Key Odorants in Hops Extracts

The volatile compounds of hops extracts were extracted by solid-phase microextraction (SPME) or solventassisted evaporative extraction (SAFE), and analyzed by gas chromatography-mass spectrometry (GC-MS). Then the aromaactive compounds were identified by gas chromatography-olfactory (GC-O) combined with aroma extraction dilution analysis (AEDA), and the key odorants were confirmed by odor activity values (OAV). Results showed that a total of 111 volatile compounds were identified by the two extraction methods combined with GC-MS. Twenty-seven aroma-active compounds were identified by GC-O/AEDA, with ethyl isobutyrate, diethyl succinate and 2-methyl butyric acid being the most abundant ones. Ethyl isobutyrate, 2-methyl butyric acid, ethyl isocaproate, ethyl octanoate, and ethyl caprate were confirmed as the key odorants of hops extracts based on flavor dilution (FD) factors and OAVs.