黑米与欧洲越橘花色苷抗氧化活性比较研究

为探究黑米与欧洲越橘花色苷抗氧化活性的差异,以抗坏血酸为阳性对照,通过比较其总还原力及清除超氧阴离子、羟自由基、过氧化氢、DPPH·、ABTS^+·的能力,对其体外抗氧化作用进行了比较研究。结果表明,在测试浓度范围内,黑米花色苷与欧洲越橘花色苷总还原能力、清除过氧化氢、DPPH·、ABTS^+·能力均高于阳性对照,且黑米花色苷具有更好地清除DPPH·、ABTS^+·能力(IC₅₀分别为0.88、0.75μg/mL);两种花色苷清除羟自由基、超氧阴离子自由基能力均低于阳性对照,但黑米花色苷清除超氧阴离子能力是欧洲越橘的3.63倍,清除羟自由基能力与欧洲越橘花色苷相当。研究表明,黑米花色苷具有更好的抗氧化性,是良好的天然抗氧化剂。

Comparative Study on Antioxidant Activity of Anthocyanins from Black Rice and European Bilberry

The objective of the present study performed a comparative analysis of the antioxidant activity of anthocyanin from black rice and bilberry.The ascorbic acid was used as a positive control.The antioxidant activities were determined by total reducing power,O2^-·,OH·,H2O2,DPPH·and ABTS+·radical scavenging methods.The results showed that total reducing power,H2O2,DPPH·and ABTS^+·radical scavenging abilities of the black rice and bilberry were better than those of the control.Furthermore,the black rice had shown the higher antioxidant activity of DPPH·and ABTS+·radical scavenging(IC50 of 0.88μg/mL for DPPH·and 0.75μg/mL for ABTS^+·).But the ability of OH·and O-2·radical scavenging were lower than those of control.While the scavenging ability of O2^-·from black rice was 3.63 times of that of bilberry,and the scavenging ability of OH·was the same as that of European bilberry.Therefore,the anthocyanin of the black rice in this study had better antioxidant properties and might be considered to be prospective natural sources of antioxidant substances.