| 丙酮-超声法提取叶绿素a工艺的优化 |
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| 引用本文： | 吴俊森,李冰,潘平.丙酮-超声法提取叶绿素a工艺的优化[J].山东建筑工程学院学报,2012(5):465-468. |
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| 作者姓名： | 吴俊森 李冰 潘平 |
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| 作者单位： | [1]山东建筑大学市政与环境工程学院,山东济南250101 [2]泰山职业技术学院,山东泰安271000 |
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| 基金项目： | 国家水体污染控制与治理科技重大专项“引黄供水地区水质风险评估技术研究与应用”（2008ZX07422-001） |
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| 摘 要： | 利用正交实验法对丙酮-超声法提取、分光光度法测定叶绿素a工艺的4个操作条件（超声功率、超声时间、离心转速、离心时间）进行优化。实验材料使用了实验室培养的小球藻，避免了藻类种类对于测定结果的影响结果表明：样品在225W功率下超声30min，6000r／min转速下离心9min，搅拌后相同条件下，重复超声、离心步骤，可以得到较好的提取效果。且环保高效，方便大批量样品的测定。
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| 关 键 词： | 叶绿素a 提取方法 正交实验法 |
Optimization of acetone-ultrasound method to extract the chlorophyll a |
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| WU Jun-sen,LI Bing,PAN Ping.Optimization of acetone-ultrasound method to extract the chlorophyll a[J].Journal of Shandong Institute of Architecture and Engineering,2012(5):465-468. |
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| Authors: | WU Jun-sen LI Bing PAN Ping |
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| Affiliation: | 1. School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, China; 2. Taishan Polytechnic,Tai＇an 271000, China) |
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| Abstract: | The four operating conditions of acetone-ultrasouno extracuon ano ~pec-ut,-u determination of chlorophyll a process （ultrasonic power, ultrasonic time, rotational speed, centrifugation time） were optimized by orthogonal experiment. The experimental material is chlorella brought up in laboratory to avoid the impact of the algae The results show that the sample sonicated at 225W power 30min, 6000r/min centrifuged 9min, and that after stirring and repeating ultrasonic and centrifugation step, it can get a better extraction effect. |
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| Keywords: | chlorophyll a extraction method orthogonal experiment |
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