海藻酸钠固定灵芝细胞对胞外三萜类产量的影响

采用海藻酸钠和氯化钙对灵芝细胞进行固定,研究包埋法固定灵芝细胞的工艺条件,探讨固定化工艺对灵芝胞外三萜类产量的影响。结果表明:固定灵芝细胞的最佳工艺条件为:4.5%海藻酸钠、2.2%CaCl2、培养基pH值自然,接种40个固定化小球,28℃、110r/min培养12d,胞外三萜类产量为(49.53±1.37)×10-2mg/mL。固定化灵芝细胞较未固定细胞,具有更好的耐酸、耐碱性,长时间培养仍具有较高的产三萜类能力,回收利用次数可达5次。

Immobilization of Ganoderma lucidum in Sodium Alginate Beads and Effect on Extracellular Triterpene Production

In this study,Ganoderma lucidum were immobilized by sodium alginate and calcium chloride.Immobilization conditions of Ganoderma lucidum using entrapment method were studied.The effects of immobilization conditions on triterpenes yield were also evaluated.The results showed the optimal conditions of immobilization of Ganoderma lucidum by sodium alginate were as follows: 4.5% of sodium alginate,2.2% of calcium chloride,nature pH,40 immobilized beads,28 ℃,110 r/min,cultured 12 days,the highest triterpenes yield was(49.53 ± 1.37)× 10-2 mg/mL.Immobilized cells had better acid and alkali resistance,higher triterpenes yield after long time culture than non-immobilized cell.It can be recycled five times.