Thymoquinone effects on DMH-induced erythrocyte oxidative stress and haematological alterations during colon cancer promotion in rats

The effect of thymoquinone (TQ), the abundant component of black cumin seed (Nigella sativa) oil extract, was evaluated on 1,2-dimethylhydrazine (DMH)-induced erythrocyte oxidative stress and haematological perturbations during colon cancer promotion in rats. Two TQ approaches, the pre- and post-treatment, were used. DMH promoted erythrocyte oxidative damage in rats by enhancing lipid peroxidation (30%) and decreasing antioxidant enzymes activities (20–35%). This was associated with the decline of erythrocyte count, haemoglobin concentration and haematocrit (20%) and the count increase of white blood cell (60%) and platelet (180%). TQ pre-treatment repaired DMH-induced erythrocyte oxidative stress, anaemia, leukocytosis, and thrombocytosis and allowed a 60% of tumour incidence decline. TQ post-treatment exerted a slight effect on erythrocyte oxidative stress and reduced colon cancer incidence by 30% only. Thus, TQ efficacy in preventing DMH-induced colon cancer promotion was related to its virtue antioxidant effect on erythrocyte oxidative stress.