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| 紫外氦氖诱变选育高产聚苹果酸菌株 | |
| 引用本文： | 乔长晟,郝华旋,姜少丽,敖爱华,王坤.紫外氦氖诱变选育高产聚苹果酸菌株[J].现代食品科技,2011,27(11):1325-1327,1331. |
| 作者姓名： | 乔长晟 郝华旋 姜少丽 敖爱华 王坤 |
| 作者单位： | 1. 天津市工业微生物重点实验室,工业微生物教育部重点实验室,天津科技大学生物工程学院,天津300457;天津北洋百川生物技术有限公司,天津300457 2. 天津市工业微生物重点实验室,工业微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 3. 天津北洋百川生物技术有限公司,天津,300457 |
| 基金项目： | 天津市高等学校科技发展基金计划(ZD200703); 科技部科技型中小企业技术创新基金(11C262111200191); 天津市科技型中小企业技术创新基金(10ZXCXSY10900) |
| 摘 要： | 以出芽短梗霉(Aureobasidium Pullulans) TKPM00006为出发菌株,用紫外和氦氖激光结合诱变,通过溴甲酚绿变色指示平板和分别以丁二酸、柠檬酸为唯一碳源的摇瓶初筛得到90株产酸明显的菌株,再经过摇床产量测定,复筛得到1株高产聚苹果酸( PMLA)的菌株.其摇瓶产量为12.62 g/L,较原菌聚苹... |
| 关 键 词： | 出芽短梗霉 聚苹果酸 诱变育种 |
Breeding of High Poly (Malic Acid)-Producing Mutation Induced by UV,He and Ne laser |
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| QIAO Chang-sheng,HAO Hua-xuan,JIANG Shao-li,AO Ai-hua,WANG Kun.Breeding of High Poly (Malic Acid)-Producing Mutation Induced by UV,He and Ne laser[J].Modern Food Science & Technology,2011,27(11):1325-1327,1331. | |
| Authors: | QIAO Chang-sheng HAO Hua-xuan JIANG Shao-li AO Ai-hua WANG Kun |
| Affiliation: | QIAO Chang-sheng1,2,HAO Hua-xuan1,JIANG Shao-li1,AO Ai-hua1,WANG Kun2(1.Tianjin Key Laboratory of Industry Microbiology,Key Laboratory of Industrial Microbiology,Ministry of Education,Tianjin University of science and technology,Tianjin 300457,China)(2.Tianjin PeiyangBiotrans Co.,Ltd,China) |
| Abstract: | In this study,ninety mutants with acid-producing capabilities were induced from Aureobasidium Pullulans TKPM00006 by UV and He Ne laser.Then they were primarily screened by Bromocresol green and the only carbon source fermentation of succinic acid and citric acid.Through the determination of poly(malic acid)-producing capability,a high poly(malic acid)-producing mutant was obtained.With this strain,PMLA yield reached 12.62g/L,which was 2.97 times than its parent strain.The results showed that the mutant was... |
| Keywords: | Aureobasidium Pullulans Poly(malic acid) Mutation breeding |
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