建立一种快速检测坂歧肠杆菌的方法

目的:建立快速检测婴儿配方奶制品(IFM)中坂歧肠杆菌的方法。方法:选择ATCC坂歧肠杆菌标准株,对不同的增菌性培养基、选择性培养基和显色培养基进行研究;结合VITEK仪和API20E细菌鉴定系统,构建快速检测配方奶制品中坂歧肠杆菌的方法。结果:建立的快速检测配方奶制品中坂歧肠杆菌的方法,所需检验流程为72h,方法灵敏度为2CUF/g,能有效区别于阴沟杆菌、产气杆菌等肠杆菌科细菌;方法应用稳定;操作简单、方法可靠,适宜规模化检测。结论:本研究认为,所建立的快速检测配方奶制品中坂歧肠杆菌的方法适宜检验检疫的工作要求,能有效地发现配方奶制品中坂歧肠杆菌的污染情况。

Rapid Method Detection of Residual E. sakazakii in Milk-based Powder Infant Formulae

Through using two step enrichment followed with isolation on chromogenic Enterobacter sakazakii agarose, a rapid method detection of Enterobacter sakazakii in milk-based powder infant formulae has been established in this research. Biochemical test systems such as API reagent or VITEK method were used to identify the suspicious colonies on the plates.The whole dctection process takes 72 hours and the sensitivity is about 2CFU/g, by which Enterobacter sakazakii can be effectively selected from other Enterobacter.This rapid method has good stability and easy operation, and is fit for the demands of inspection and quarantine.