茶薪菇酚类提取物的抗氧化功效评价

目的:研究茶薪菇提取物的酚类物质对体外和体内抗氧化活性的影响。方法:在分析茶薪菇水提物、丙酮提取物、乙醇提取物的总酚、总糖含量以及体外抗氧化的基础上,以阿魏酸、维生素E为阳性对照组,以生理盐水为空白对照组,检测连续灌胃10 d茶薪菇水提物以总酚含量计,12 mg/(kg bw·d)]对正常小鼠的抗氧化功效,采用D-半乳糖衰老小鼠模型评价茶薪菇水提物以总酚含量计,12,20,40 mg/(kg bw·d)]对衰老小鼠的抗氧化功效。结果:茶薪菇水提物的总酚含量为1.25‰,介于丙酮提取物和乙醇提取物之间,当茶薪菇水提物质量浓度为60.00 mg/mL时,对羟自由基的清除率高达87.00%,对卵黄脂蛋白不饱和脂肪酸过氧化的抑制率高达65.31%。茶薪菇水提物对正常小鼠的抗氧化结果显示:茶薪菇水提物不影响正常小鼠体重增长,有调高肝脏指数和脾脏指数的趋势,茶薪菇水提物组的超氧化物歧化酶(SOD)活力最高达130.43 U/mL;丙二醛(MDA)含量明显低于生理盐水对照组(P<0.05),抗氧化能力与维生素E和阿魏酸相当。茶薪菇水提物对衰老小鼠的抗氧化结果显示:与D-半乳糖诱导的衰老小鼠比较,10,20,40 mg/(kg bw·d)的茶薪菇水提物极显著降低了衰老小鼠的MDA含量(P<0.01),20 mg/(kg bw·d)剂量组下降率高达94.53%;10 mg/(kg bw·d)剂量组谷胱甘肽过氧化物酶(GSH-Px)活力极显著提高22.98%(P<0.01)。结论:茶薪菇水提物中酚类物质是体外和体内抗氧化的主要功效成分,通过提高SOD、GSH-Px等抗氧化酶活力,降低MDA含量,发挥维持机体抗氧化稳态和拮抗D-半乳糖诱导的衰老损伤的作用。

Evaluation of Antioxidant Activity of Phenolic Components from Agrocybe chaxingu Huang

Objective:The phenolic substances in the extract of Agrocybe chaxingu Huang and its effect on antioxidant activity in vivo and in vitro were studied.Method:Based on the analysis of total phenols,total sugars and antioxidant activities in vitro of water/acetone/ethanol extract of A.chaxingu,the antioxidant activity of A.chaxingu aqueous extracttotal phenols,12 mg/(kg bw·d)]on normal mice(gavaged for 10 days)was detected,which made ferulic acid and vitamin E as positive control group,and physiological saline as blank control group.Then,the antioxidant activity of A.chaxingu aqueous extractbased on total phenols,10,20,40 mg/(kg bw·d)]on the D-galactose induced aging mice was evaluated.Result:The content of total phenols of A.chaxingu aqueous extract was 1.25‰,which between acetone extract and ethanol extract.When the concentration of A.chaxingu aqueous extract was 60.00 mg/mL,the scavenging rate of hydroxyl radicals and the inhibition rate of polyunsaturated fatty acid(PUFA)were 87.00%and 65.31%,respectively,which were more higher than acetone extract and ethanol extract.The results of antioxidant activity on normal mice showed that the A.chaxingu aqueous extract did not affect the weight growth,but increased the liver index and spleen index.The activity of SOD in serum of A.chaxingu aqueous extract increased and reached 130.43 U/mL,while the content of MDA decreased obviously compared with the physiological saline group(P<0.05).Antioxidant capacity of,aqueous extract was equivalent with vitamin E and ferulic acid.The results of antioxidant activity on D-galactose induced aging mice showed that 10,20,40 mg/(kg bw·d)of A.chaxingu aqueous extract could significantly decreased the content of MDA(P<0.01),and the decrease rate of MDA of 20 mg/(kg bw·d)group was the highest and reached 94.53%.The GSH-Px activity of 10 mg/(kg bw·d)group increased extremely significantly 22.98%(P<0.01).Conclusion:The A.chaxingu aqueous extract had antioxidant activity in vivo and in vitro,and phenolic substances were the main functional components.A.chaxingu aqueous extract could maintain the body's antioxidant homeostasis and significantly amelioration the D-galactose induced aging damage by increasing the activity of antioxidant enzymes(SOD,GSH-Px)and reducing the content of MDA.