基于鼠精细胞的味觉阻抗传感器 用于苦味物质检测的研究

本文设计了一种基于雄鼠精细胞的细胞阻抗传感器用于苦味物质的特异性检测.雄鼠精细胞内含有大量T2Rs受体(G蛋白偶联受体)可以对苦味物质产生特异性响应,苦味受体被苦味物质激活后产生的响应会引起细胞形态骨架的变化,从而可以被细胞阻抗传感器检测.本文探索了实验的最佳细胞密度,检测了苯硫脲和奎宁两种常见的苦味物质的响应,苯硫脲检测范围为10μmol/L~200μmol/L,检出限为4μmol/L;奎宁检测范围为62.5μmol/L~1000μmol/L,检出限为40μmol/L.传感器阻抗值增量与苦味物质浓度呈一定的线性相关性.此外,论文对该味觉传感器的特异性进行了测试分析,表明了基于细胞传感器的检测方法为代替动物和人的苦味测试提供了一种可能的新的手段.

A study on mouse germ cell-based sensor for the detection of bitterness

In this study,the feasibility of utilizing male mouse germ cells in testis and electrical cell-substrate imped?ance sensor(ECIS)was explored to build a cell-based bitter biosensor,which can sensitively and specifically respond to bitter compounds. Male mouse germ cells express lots of bitter receptor T2Rs(G protein coupled receptors)which can sensitively and specifically respond to bitter compounds,when active by ligands will affect cell morphology in a specific manner. The best cell density was explored;cell-impedance response profiles of germ cells to two bitter com?pounds were investigated by analyzing the response intensity under various concentrations,The linear detection range of PTC is 10μmol/L~200μmol/L,the detection limit is 4μmol/L;the linear detection range of quinine is 62.5μmol/L~1000μmol/L,the detection limit is 4μmol/L; Furthermore,impedance responses to five basic tastes were exam?ined to evaluate the specificity of cell-based bitter biosensor in bitter detection. The results revealed that this hybrid bitter biosensor could respond to those bitter compounds in a dose-dependent manner. And it could respond to bitter compounds specifically. This biosensor may provide a promising approach for detecting various bitter compounds.