Biohydrogen using leachate from an industrial waste landfill as inoculum |
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Authors: | Hisanori Watanabe Hidekichi Yoshino |
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Affiliation: | 1. Kanagawa Environmental Research Center, Shinomiya1-3-39, Hiratsuka, Kanagawa 254-0014, Japan;2. Institute of Environment and Resource Systems, Moegino11-23, Aobaku, Yokohama 227-0044, Japan |
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Abstract: | Since hydrogen is a renewable energy source, biohydrogen has been researched in recent years. However, there is little data on hydrogen fermentation by a leachate from a waste landfill as inoculum. We investigated hydrogen production using a leachate from an industrial waste landfill in Kanagawa prefecture. The results showed no methane gas production and the leachate was a suitable inoculum for hydrogen fermentation. The maximum H2 yield was 2.67 mol of H2 per mol of carbohydrate added, obtained at 30 °C and initial pH 7. The acetate and butyrate production was significant when the H2 yield was higher. The oxidation–reduction potential analysis of the culture suggested that hydrogen-producing bacteria in the leachate were facultatively anaerobic. Scanning electron microscope observations revealed hydrogen-producing bacteria comprised bacilli of about 2 μm in length. |
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