| 超临界CO_2萃取结合柱色谱分离萝芙木生物碱 |
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| 引用本文： | 周雪晴,冯玉红,张冲,林强.超临界CO_2萃取结合柱色谱分离萝芙木生物碱[J].精细化工,2007,24(2):154-155,161. |
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| 作者姓名： | 周雪晴 冯玉红 张冲 林强 |
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| 作者单位： | 海南大学,理工学院,海南大学热带生物资源教育部重点实验室,海南,海口,570228 |
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| 摘 要： | 用超临界CO2萃取技术,设计了4因素3水平正交实验,研究从海南催吐萝芙木中提取利血平生物碱的最佳工艺。最佳提取条件为:萃取压力35 MPa,萃取温度60℃,夹带剂为100 g样品用25 mL乙醇,萃取时间2 h。用高效液相色谱法对萃取产物中的利血平含量进行了测定。将萃取所得产物用硅胶柱色谱进一步分离提纯,得到了质量分数为99.8%的利血平针状结晶。
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| 关 键 词： | 超临界CO2萃取 柱色谱 利血平 |
| 文章编号： | 1003-5214（2007）02-0154-03 |
| 修稿时间： | 2006-07-292006-10-12 |
Extraction of Reserpine from Hainan Rauvolfia with Supercritical CO2 |
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| ZHOU Xue-qing,FENG Yu-hong,ZHANG Chong,LIN Qiang.Extraction of Reserpine from Hainan Rauvolfia with Supercritical CO2[J].Fine Chemicals,2007,24(2):154-155,161. |
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| Authors: | ZHOU Xue-qing FENG Yu-hong ZHANG Chong LIN Qiang |
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| Affiliation: | Science and Engineering School, Hainan University, Key Lab. of Tropic Biological Resource of Chinese Education Ministry, Haikou 570228, Hainan, China |
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| Abstract: | A new extraction process of reserpine from Hainan Rauvolfia with supercritical CO_2 fluid extraction(SFE) was studied in detail.The optimum extraction process conditions include keeping pressure at 35 MPa and temperature at 60 ℃ for 2 h,in the same time adding 25 mL alcohol per 100 g raw materials.The content of reserpine in the extract was tested by HPLC.The extract was further purified by column chromatography to get the acicular crystals of reserpine of 99.8% purity. |
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| Keywords: | supercritical CO2 extraction column chromatography reserpine |
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