| 米曲霉HDF-7蛋白酶的分离纯化及酶学性质研究 |
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| 引用本文： | 于艳颖,董海莉,平文祥,葛菁萍. 米曲霉HDF-7蛋白酶的分离纯化及酶学性质研究[J]. 黑龙江水专学报, 2011, 2(2): 74-79 |
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| 作者姓名： | 于艳颖 董海莉 平文祥 葛菁萍 |
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| 作者单位： | 黑龙江大学,生命科学学院,哈尔滨,150080;黑龙江大学,微生物黑龙江省重点实验室,哈尔滨,150080 |
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| 基金项目： | 黑龙江省科技攻关重大项目，功能微生物选育及其产物应用研究创新团队 |
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| 摘 要： | 对米曲霉 HDF-7 所产蛋白酶进行分离纯化,经硫酸铵沉淀和凝胶过滤层析得到电泳纯的蛋白酶,经SDS-PAGE 电泳测定其相对分子质量约为30 KDa.该酶的最适反应温度为50℃,最适作用pH值为7.0,该酶在20℃时具有良好的热稳定性,在pH6.0～8.0的条件下酶是相对稳定的,Mn2+对该蛋白酶有明显的激活作用,Cu2+对该蛋白酶有强烈的抑制作用,Km值为78μg/mL,最大反应速度V max为6.29μg/min.
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| 关 键 词： | 米曲霉 蛋白酶 分离纯化 酶学性质 |
Studies on purification and properties of protease produced by Aspergillus oryae HDF-7 |
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| YU Yan-ying,DONG Hai-li,PING Wen-xiang,GE Jing-ping. Studies on purification and properties of protease produced by Aspergillus oryae HDF-7[J]. Journal of Heilongjiang Hydraulic Engineering College, 2011, 2(2): 74-79 |
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| Authors: | YU Yan-ying DONG Hai-li PING Wen-xiang GE Jing-ping |
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| Affiliation: | a,b(Heilongjiang Province Key Laboratory of Microbiology,College of Life Science,Heilongjiang University,Harbin 150080,China) |
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| Abstract: | The protease produced by Aspergillus oryzae HDF-7 was purified by using ammonium sulfate precipitations and gel filtration chromatography.The purified protease showed on SDS-PAGE as a single band with an apparent molecular weight of 30 KDa.The optimum temperature was 50℃ and the optimum pH was 7.0;It was found that the protease was stable at 20 ℃ and at pH 6.0-8.0;Protease activity was strongly activated by Mn2＋ and strongly inhibited by Cu2＋.The Km values of the protease for casein at 40 ℃ was 78 μg /mL with Vmax 6.29 μg/min. |
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| Keywords: | Aspergillus oryzae the protease purification enzymatic properties |
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