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Engineering a mammalian super producer
Authors:Stefanie Dietmair  Lars K. Nielsen  Nicholas E. Timmins
Affiliation:Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, 4072 QLD, Australia
Abstract:Mammalian cells are the preferred host for the manufacture of a wide range of biopharmaceuticals, but production costs are high owing to low productivity. A range of rational engineering strategies have been pursued in order to increase volumetric product titres from mammalian cells, such as delaying apoptosis, manipulation of the cell cycle, and improving metabolism and protein processing. Unfortunately, outcomes from these strategies have been mixed, with few instances where significant improvements in product yield have been achieved. This article reviews and contrasts many of the engineering strategies attempted to date, highlighting the variability and context specificity in outcome. The paper argues that this is a reflection of the complexity of mammalian cells, and that a deeper understanding of the biology underpinning protein production for biotechnological purposes is required. Copyright © 2011 Society of Chemical Industry
Keywords:mammalian cell  host cell engineering  review
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