心肌组织传感阵列在信号传导及药物分析中的应用

为了对心肌组织的搏动信号及其传导进行实时监测与分析,设计一种用于离体心肌组织电生理检测的组织传感阵列.根据组织/器件耦合原理,该传感阵列能够检测伴随心肌细胞搏动产生的场电位的变化.利用组织传感阵列,同步采集多通道的心肌信号并观察心血管药物盐酸肾上腺素和氯化乙酰胆碱对搏动信号的影响.实验结果显示：离体心肌组织及切片表现出良好的搏动和信号传导；盐酸肾上腺素和氯化乙酰胆碱对心肌组织搏动信号的幅度、频率、持续时间甚至信号的传导分别表现出兴奋和抑制作用.说明组织传感阵列技术对离体心肌组织搏动信号的提取是可行的,并且对心肌信号的传导及药物作用分析非常有用.

Cardiac tissue-based sensing array for studying on signal conduction and drugs analysis

To real-time monitor the beating signals of the cardiac tissues and investigate the signal propagation,an in vitro cardiac tissue-based sensing array was developed for monitoring the electrophysiological activities.According to the coupling principle between tissues and sensors,the sensing array can detect the changes of the field potentials with the mechanical beatings of the cardiac cells.With the tissue-based sensing array,the multi-channel data was acquired synchronously and the effect of Adrenaline Hydrochloride and Acetylcholine Chloride on the beating signals was observed.Experiment results showed that the in vitro cardiac tissues and slices took on good beatings as well as the propagation.Adrenaline Hydrochloride and Acetylcholine Chloride respectively had exciting and relaxing action on signals’ amplitude,frequency,duration and even the signal conduction.In conclusion,the tissue-based sensing array technique is feasible to measure the beating signals of the in vitro cardiac tissues,and is quite advantageous to study the signal conduction and drugs screening.