ns脉冲电场发生器的研制及应用

为了研究ns脉冲电场诱导肿瘤细胞的凋亡效应,结合开关电源技术和脉冲功率技术,研制了一套多参数大范围独立可调的ns脉冲电场发生器。该发生器由高压直流电源、脉冲形成电路和辅助电路3部分组成,输出的脉冲幅值0～1.2kV内连续可调,重复频率1Hz～10kHz内连续可调,脉冲宽度分100、200、600和1000ns4级可调,脉冲个数可以任意预置,上升时间<30ns,并具有显示和保护功能。实验室调试和医学细胞实验结果表明,该发生器调节方便、性能稳定,能够有效诱导人肝癌细胞凋亡,为ns脉冲电场诱导肿瘤细胞凋亡的机理和阈值参数选择规律的深入研究奠定了基础。

Development and Application of Nanosecond Pulsed Electric Fields Generator

In order to research apoptosis effects of cancer cell induced by nanosecond pulsed electric fields (nsPEF), on the basis of combination of switching power supply technology with pulsed power technology, this paper developed a nsPEF generator whose output multiple parameters could be adjusted in a wide range and independently. The generator was composed of a high-voltage direct current power supply, a pulse forming circuit and an assistant circuit. The amplitude of output pulse could be adjusted continually from 0 V to 1.2 kV by means of adjusting the driving signal in high-voltage direct current power supply. The repeat rate of output pulse could be adjusted continually from 1 Hz to 1 kHz by means of adjusting the control circuit in assistant circuit. The width of output pulse could be adjusted to 100ns, 200 ns, 600 ns and 1 000 ns by means of switching to different energy storing capacitor in pulse forming circuit. The number of output pulse could be preset to any value and the rise time of output pulse was less than 30 ns. All above-mentioned parameters could be displayed via LED. Over-voltage, over-current and short circuit protections were also available. Debugging results in laboratory and medical cell experiment results show that nsPEF generator can be adjusted conveniently and operated stably. Furthermore, nsPEF can induce apoptosis of human liver cancer cell effectively. In conclusion, this nsPEF generator is competent for research of mechanism and optimal parameters for cancer cell apoptosis induction.