| 不同搅拌速度对松口蘑菌丝体发酵的影响 |
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| 引用本文： | 魏元青,许正宏,许泓瑜,张宏斌,陶文沂.不同搅拌速度对松口蘑菌丝体发酵的影响[J].食品与发酵工业,2005,31(8):134-136. |
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| 作者姓名： | 魏元青 许正宏 许泓瑜 张宏斌 陶文沂 |
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| 作者单位： | 江南大学生物工程学院,无锡,214036 |
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| 基金项目： | 江苏省无锡市自然科学基金资助课题（No.CK030002） |
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| 摘 要： | 在摇瓶发酵条件优化的基础上,研究了松口蘑在25L搅拌式发酵罐上的分批放大实验,探讨了不同搅拌转速对菌丝生物量、生产强度、pH、溶氧及对菌丝形态的影响,在发酵温度25℃,通风量0.8vvm,罐压0.5kg/cm2的条件下,最佳搅拌转速为100r/min。发酵培养8d后,发酵液由浑浊变为澄清,上清液亮黄色,菌丝形态呈均匀颗粒状,菌丝生物量增加趋于平缓,最高可达11.5g/L。
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| 关 键 词： | 松口蘑 深层发酵 搅拌转速 |
| 收稿时间： | 03 3 2005 12:00AM |
| 修稿时间： | 2005年3月3日 |
Study on Submerged Culture of Tricholoma Matsutake |
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| Wei Yuanqing,Xu Zhenghong,Xu hongyu,Zhang Hongbin,Tao Wenyi.Study on Submerged Culture of Tricholoma Matsutake[J].Food and Fermentation Industries,2005,31(8):134-136. |
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| Authors: | Wei Yuanqing Xu Zhenghong Xu hongyu Zhang Hongbin Tao Wenyi |
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| Abstract: | On the basis of flask fermentation of Tricholoma Matsutake, it was cultured by 25 L-fermentor. The biomass of mycelium, dissolved oxygen, pH and the shape of mycelium were studied on the influence of different rotors. The experimental results showed that the best rotating speed was 100 r/min when the temperature was 25℃, the volume ratio of flow air was 0.8vvm and the pressure was 0.5 kg/cm2. After 8 days, the broth was transparent and yellow, the mycelium was round pellet, the biomass of mycelium reached the highest lever which was 11.5g/l. |
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| Keywords: | Tricholoma Matsutake submerged culture rotating speed |
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