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Zeolite‐Coated Porous Arrays: A Novel Strategy for Enzyme Encapsulation
Authors:V R Reddy Marthala  Mona Friedrich  Zhou Zhou  Monica Distaso  Stephanie Reuss  Shaeel A Al‐Thabaiti  Wolfgang Peukert  Wilhelm Schwieger  Martin Hartmann
Affiliation:1. Erlangen Catalysis Resource Center (ECRC), Friedrich‐Alexander‐Universit?t Erlangen‐Nürnberg, Erlangen, Germany;2. Institute of Particle Technology, Friedrich‐Alexander‐Universit?t Erlangen‐Nürnberg, Erlangen, Germany;3. Institute of Chemical Reaction Engineering, Friedrich‐Alexander‐Universit?t Erlangen‐Nürnberg, Erlangen, Germany;4. Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
Abstract:Zeolite Beta‐coated stainless steel supports with gradient porosity are employed as filter‐panels for lipase encapsulation. Enzyme encapsulation on the stainless steel porous discs is achieved via vacuum infiltration. Subsequently, two lipase‐encapsulated zeolite Beta‐coated stainless steel discs are attached using an adhesive. The zeolite Beta layer on the stainless steel discs largely prevents lipase leaching in comparison to the stainless steel discs without a zeolite layer. The activity of the lipase‐encapsulated, attached zeolite Beta‐coated stainless steel porous discs depends on the thickness of the zeolite Beta layer. It is shown that the biocatalytic performance of the lipase‐encapsulated, attached zeolite Beta‐coated stainless steel supports with a zeolite Beta layer thickness of ≈0.7–1 μm is better than the lipase‐encapsulated, attached zeolite Beta‐coated stainless steel supports with a zeolite Beta layer thickness of ≈2–3 μm in the lipase‐catalyzed transesterification of vinyl propionate with 1‐butanol using n‐hexane as solvent.
Keywords:encapsulation  enzymes  lipases  stainless steel membranes  zeolites
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