水母雪莲愈伤组织超低温保存条件的初探

初步研究了水母雪莲愈伤组织的超低温保存方法. 结果表明，预培养、保护剂、预处理、冰冻后处理对愈伤组织存活率都有一定影响. 水母雪莲愈伤组织预培养基为添加5%二甲基亚砜的MS培养基，优化的冰冻保护剂是15%二甲基亚砜+15%乙二醇+30%甘油的0.4 mol/L蔗糖液，冰冻保护剂的预处理温度是15℃，时间为10 min，解冻温度25~35℃；在25℃水浴中用含1.2 mol/L蔗糖的MS溶液反复洗3次，每次10 min. 用TTC法测定细胞存活率可达58.5%.

Preliminary Research on Cryopreservation of Saussurea medusa Maxim Callus

The cryopreservation condition of Saussurea medusa callus was studied. The results indicated that preculture, cryoprotectant, pretreatment, recovery and washing conditions affected the survival rate of Saussurea medusa callus. The preculture medium was MS solid culture medium supplemented with 5% dimethyl sulfoxide. The optimum composition of cryoprotectant was 15% ethyleneglycol, 15% simethyl sulfoxide, 30% glycerol and 0.4 mol/L sucrose. The pretreatment temperature and time were 15oC and 10 min, respectively. The recovery temperature was 25~40oC. The survival rate of Saussurea medusa callus was 58.5% after washed by MS liquid medium with 1.2 mol/L sucrose for 3 times (10 min for each time ) at 25oC.