Semi-continuous Hydrolysis of Sweet Potato Raw Starch by Chalara paradoxa Glucoamylase |
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| Authors: | TAKAHIRO NODA TOSHIO OHTANI TAKEO SHIINA YOSHIHIKO NAWA |
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| Affiliation: | Author Noda is with Kyushu National Agricultural Experiment Station, Ministry of Agriculture, Forestry &Fisheries, Suya 2421, Nishigoshi, Kumamoto, 861-11 Japan. Author Ohtani is with National Research Institute of Agricultural Engineering, Ministry of Agriculture, Forestry and Fisheries, Kannondai 2-1-2, Tsukuba, Ibaraki, 305 Japan. Author Shiina is with Hokkaido National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries, Memuro, Kasai-gun, Hokkaido, 082 Japan. Address inquiries to Dr. Y. Nawa. |
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| Abstract: | Effect of preservatives was investigated on semi-continuous digestion of sweet potato raw starch by Chalara paradoxa glucoamylase which digests both raw and gelatinized starch. Potassium metabisulfite strongly inhibited raw starch digesting activity (RDA) although gelatinized starch digesting activity was only slightly inhibited. Potassium sorbate, among the preservatives examined, showed the weakest inhibition (below 10%) on RDA. In the presence of potassium sorbate, semi-continuous digestion of sweet potato raw starch in a 20L scale hollow fiber UF membrane pilot reactor proceeded efficiently at 30% (w/v) initial concentration of raw starch at pH 5.0 and 40°C. Glucose production was 5.7 to 6.0 g/hr/L. |
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| Keywords: | starch sweet potato hollow fiber reactor |
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